PFUPISO UYE TSANANGURO MUYEdzo
Manyoka ndechimwe chezvikonzero zvinokonzeresa kurwara nekufa kwevana pasi rose, zvichikonzera kufa kwemamiriyoni maviri nemazana mashanu pagore.Utachiona hweRotavirus ndiyo inonyanya kukonzera manyoka akanyanya mucheche nevana vari pasi pemakore mashanu, inoverengera 40% -60% yeacute gastroenteritis uye ichikonzera kufa kunofungidzirwa kwe500,000 kwevana gore rega rega.Pakazosvika makore mashanu, anenge mwana wese pasi rose akatapukirwa nerotavirus kamwechete.Nezvirwere zvinotevera, mhinduro yakafara, heterotypic antibody inokurudzirwa;saka, vanhu vakuru havawanzobatwa.
Kusvika iye zvino mapoka manomwe e rotaviruses (mapoka AG) akaparadzaniswa uye
characterized.Boka reRotavirus, iro rinonyanyozivikanwa, rinokonzera zvinopfuura makumi mapfumbamwe kubva muzana zvezvirwere zvese zveRotavirus muvanhu.Rotavirus inotapurirwa kunyanya nefecaloral nzira, zvakananga kubva kumunhu kuenda kumunhu.Virus titers mustool inosvika pakakwirira nguva pfupi mushure mekutanga kwechirwere, yobva yaderera.Iyo incubation nguva yehutachiona hwe rotavirus inowanzova zuva rimwe kusvika matatu uye inoteverwa negastroenteritis ine avhareji yemazuva matatu kusvika manomwe.Zviratidzo zvechirwere ichi kubva pakunyorova, manyoka ane mvura kusvika pakuita manyoka akanyanya nefivha uye kurutsa.
Kuongororwa kwehutachiwana hwe rotavirus kunogona kuitwa mushure mekuongororwa kwegastroenteritis sechikonzero chekunyanya kuita manyoka muvana.Munguva pfupi yapfuura, kuongororwa kwakananga kwehutachiona ne rotavirus kwave kuwanikwa kuburikidza nekuonekwa kwehutachiona antigen muchituru nemaitiro eimmunoassay akadai se latex agglutination assay, EIA, uye lateral flow chromatographic immunoassay.
Rotavirus Ag Rapid Test is a lateral flow chromatographic immunoassay iyo inoshandisa maviri emasoja ekudzivirira chirwere kuti akwanise kuona rotavirus antigen mufecal specimen.Iyo bvunzo inogona kuitwa pasina inorema murabhoritari michina, uye mhedzisiro inowanikwa mukati memaminitsi gumi nemashanu.
PRINCIPLE
Iyo Rotavirus Ag Rapid Test ndeye lateral kuyerera chromatographic immunoassay.Mutambo wekuyedza unosanganisira: 1) burgundy colored conjugate pad ine monoclonal anti-rotavirus antibody yakasanganiswa ne colloidal goridhe (anti-rotavirus conjugates) uye inodzivirira antibody yakabatanidzwa ne colloidal goridhe, 2) nitrocellulose membrane strip ine mutsara webvunzo (T. mutsara) uye mutsara wekutonga (C mutsara).Mutsara weT wakagara wakanamirwa neimwe monoclonal anti-rotavirus antibody, uye C mutsara wakagara wakaputirwa neine control line antibody.
Kana huwandu hwakakwana hwemuenzaniso wakabviswa ukaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito pakaseti.Rotavirus Ag, kana iripo mumuenzaniso, ichasunga kune anti-rotavirus conjugates.Iyo immunocomplex inobva yatorwa pa membrane ne pre-coated rotavirus antibody inogadzira burgundy colored T line, ichiratidza rotavirus positive test result.Kusavapo kweT line kunoratidza kuti mutsara we rotavirus Ag mumuenzaniso uri pasi pezinga rinoonekwa, zvichiratidza mhedzisiro isina kunaka ye rotavirus.Iyo bvunzo ine mukati mekutonga (C mutsara), iyo inofanirwa kuratidza burgundy ine ruvara mutsara weiyo immunocomplex yekutonga antibodies, zvisinei nekukura kwemavara pamutsara weT.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
