PFUPISO UYE TSANANGURO MUYEdzo
Zika virus (Zika): inonyanya kutapuriranwa nekurumwa nemosquito yeAedes, amai nemwana, kuwedzerwa ropa uye kutapurirana pabonde. Nekuti parizvino hapana mushonga wekudzivirira, vanhu vanowanzobatwa neutachiona.IgG/IgM antibody inogadzirwa vhiki imwe mushure mekutanga, saka kuonekwa kweIgG/IgM kwakakosha zvikuru kwekutanga.
kuongororwa kweZika virus.Zika inoongororwa zvichienderana nekuongorora serological uye kuparadzaniswa kwevhairasi mumakonzo kana matishu tsika.IgM immunoassay ndiyo inonyanya kushanda yebvunzo nzira.Iyo zika IgM/IgG Rapid Test inoshandisa recombinant antigens inotorwa kubva kune yayo chimiro mapuroteni, inoona IgM/IgG anti-zika mumurwere serum kana plasma mukati memaminitsi gumi nemashanu.Muedzo unogona kuitwa nevashandi vasina kudzidziswa kana hunyanzvi hushoma, vasina midziyo yemurabhoritari inorema.
PRINCIPLE
Iyo Zika IgM/IgG Rapid Test is a lateral flow chromatographic immunoassay.Iyo bvunzo kaseti ine: 1) burgundy yeruvara conjugate pad ine recombinant antigen yakasanganiswa negoridhe colloid (Zika conjugates) uye tsuro IgG-goridhe conjugates, 2) nitrocellulose membrane strip ine maviri ebvunzo mabhendi (M uye G mabhendi) uye yekudzora. bhendi (C bhendi).Iyo M band yakagara yakavharwa ne monoclonal anti-human IgM yekuona IgM anti-Zika, G bhendi rakafanoiswa reagents kuti rionekwe IgG anti-Zika, uye C band rakafanoputirwa nembudzi anti. tsuro IgG.
Kana vhoriyamu yakakwana yebvunzo yemuenzaniso yaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.Anti-Zika IgM kana iripo mumuenzaniso inosunga kune Zika conjugates.Iyo immunocomplex inozobatwa pa membrane neiyo pre-coated anti-human IgM antibody, ichigadzira burgundy ine ruvara rweM bhendi, ichiratidza Zika IgM yakanaka bvunzo mhedzisiro.
Anti-Zika IgG kana iripo mumuenzaniso inosunga kune Zika conjugates.Iyo immunocomplex inozotorwa neiyo pre-yakavharwa reagents pa membrane, ichigadzira burgundy yemavara G bhendi, ichiratidza Zika IgG yakanaka bvunzo mhedzisiro.Kusavapo kwechero mabhendi ebvunzo (M naG) kunoratidza mhedzisiro yakaipa.Iyo bvunzo ine yemukati kudzora (C bhendi) iyo inofanirwa kuratidza burgundy ruvara bhendi ye immunocomplex yembudzi anti rabbit IgG/tsuro IgG-goridhe conjugate zvisinei nekusimudzirwa kwemavara pane chero mabhendi ekuedzwa.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
