PFUPISO UYE TSANANGURO MUYEdzo
Helicobacter pylori inobatanidza nezvirwere zvakasiyana-siyana zvepamuviri zvinosanganisira kwete-ulcer dyspepsia, duodenal uye gastric ulcers uye inoshanda, isingagumi gastritis.Kuwanda kwehutachiona hweH. pylori hunogona kudarika 90% kune varwere vane zviratidzo uye zviratidzo zvezvirwere zvemudumbu.Zvidzidzo zvenguva pfupi yapfuura zvinoratidza kubatana kweH. pylori utachiona nekenza yemudumbu.
pylori inogona kutapurirwa kuburikidza nekudya kwekudya kana mvura yakasvibiswa nefecal matter.Mishonga inorwisa mabhakitiriya pamwe chete nemisanganiswa yebismuth yakaratidzwa kuva inoshanda mukurapa hutachiona hweH. Pylori..H.pylori utachiona parizvino hunoonekwa nenzira dzekuedza dzinopinda dzinobva paendoscopy uye biopsy (kureva histology, tsika) kana nzira dzisiri dzekuyedza dzakaita seurea breath test (UBT), serologic antibody test uye stool antigen test.UBT inoda midziyo yerabhu inodhura uye kushandiswa kweradioactive reagent.Serologic antibody bvunzo haisiyanise pakati pehutachiona huri kushanda uye kuratidzwa kwakapfuura kana hutachiona hwakarapwa.Iyo stool antigen test inoona antigen iripo mutsvina, iyo inoratidza inoshanda H. pylori utachiona.Inogonawo kushandiswa kutarisa kushanda kwekurapa uye kudzoka kwechirwere.H. pylori Ag Rapid Test inoshandisa colloidal gold conjugated monoclonal anti-H.pylori antibody uye imwe monoclonal anti-H.pylori antibody kuti inyatsoona H. pylori antigen iripo mufecal specimen yemurwere ane utachiona.Muedzo uri mushandisi, wakarurama, uye mhedzisiro inowanikwa mukati memaminitsi gumi nemashanu.
PRINCIPLE
H. pylori Ag Rapid Test is a sandwich lateral flow chromatographic immunoassay.Mutambo wekuyedza unosanganisira: 1) burgundy colored conjugate pad ine monoclonal anti-H.pylori antibody yakabatanidzwa negoridhe colloidal (anti-Hp conjugates) uye 2) nitrocellulose membrane strip ine test line (T line) uye control line (C line).Mutsara weT wakafanoputirwa neimwe monoclonal anti-H.pylori antibody, uye C mutsetse wakagara wakaputirwa nembudzi anti-mbeva IgG antibody.
Kana vhoriyamu yakakwana yefecal specimen yakaburitswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito pakaseti.H. pylori antigens, kana iripo mumuenzaniso, ichasunga kune anti-Hp conjugates.Immunocomplex inobva yatorwa pa membrane ne-pre-coated antibody inogadzira burgundy colored T line, ichiratidza H. pylori positive test result.Kusavapo kwemutsara weT kunoratidza kuti huwandu hweH. pylori antigens mumuenzaniso uri pasi pezinga rinoonekwa, richiratidza H. pylori negative test result.Muedzo une mukati mekutonga (C line) iyo inofanira kuratidza burgundy colored line ye iyo immunocomplex yembudzi anti-mouse IgG/mouse IgG-goridhe conjugate zvisinei nekukura kwemavara pamutsara weT.Kana C line ikasakura, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa neimwe mudziyo.
