PFUPISO UYE TSANANGURO MUYEdzo
Lymphatic filariasis inozivikanwa seElephantiasis, inonyanya kukonzerwa neW. bancrofti naB. malayi, inobata vanhu vanenge mamiriyoni 120 munyika dzinopfuura 80.Chirwere ichi chinotapuriranwa kuvanhu nekurumwa neumhutu hune utachiona mukati umo microflariae inoyamwa kubva kune ine hutachiona hwemunhu inokura kuita nhanho yetatu.Kazhinji, kudzokorora uye kwenguva refu kuratidzwa kune utachiona hwemakonye kunodiwa pakugadzwa kwehutachiona hwevanhu.
The definitive parasitologic diagnosis ndiyo kuratidzwa kwe microflariae mumasampuli eropa.Nekudaro, iyi chiyero chegoridhe bvunzo inoganhurirwa nezvinodiwa zvekuunganidza ropa kwehusiku uye kushaikwa kwekunzwa kwakakwana.Kuonekwa kwemaantigen anotenderera kunowanikwa mune zvekutengesa.Kubatsira kwayo kunogumira kuW. bancrofti.Mukuwedzera, microfilaremia uye antigenemia inokura kubva pamwedzi kusvika kumakore mushure mekuratidzwa.
Kuonekwa kwemasoja ekudzivirira chirwere kunopa nzira yekutanga kuona filarial parasite utachiona.Kuvapo kweIgM kune maantigen eparasite kunoratidza hutachiona hwazvino, nepo, IgG inoenderana nedanho rekupedzisira rehutachiona kana hutachiona hwekare.Uyezve, kuzivikanwa kwemaantigen akachengetedzwa kunobvumira 'pan-filaria' bvunzo kuti ishande.Kushandisa recombinant mapuroteni kunobvisa kusangana nevanhu vane zvimwe zvirwere zveparasitic.
Iyo Filariasis IgG/IgM Combo Rapid Test inoshandisa yakachengetedzwa recombinant
maantigens kuona panguva imwe chete IgG neIgM kune W. bancrofti uye B. malayi tupukanana pasina kurambidzwa kuunganidzwa kwemuenzaniso.
PRINCIPLE
Iyo Filariasis IgG/IgM Rapid Test Kit is a lateral flow chromatographic immunoassay.The test cassette inosanganisira: 1) burgundy colored conjugate pad ine recombinant W. bancrofti uye B. malayi common antigens conjugated ne colloid gold (Filariasis conjugates) uye rabbit IgG-gold conjugates, 2) nitrocellulose membrane strip ine mabhendi maviri ekuedzwa (C) uye mabhendi maviri ekuedzwa.Iyo M band yakavharwa nemonoclonal anti-human IgM yekuona IgM anti- W. bancrofti uye B. malayi, G bhendi rakafanoputirwa nemareagents ekuona IgG anti-W.bancrofti uye B. malayi, uye C band rakafanoputirwa nembudzi anti tsuro IgG.
Kana vhoriyamu yakakwana yemuenzaniso webvunzo yaiswa mumuenzaniso tsime rekaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.W. bancrofti kana B. malayi masoja ekudzivirira chirwere eIgM kana aripo mumuenzaniso anosunga kune Filariasis conjugates.Iyo immunocomplex inobva yatorwa pa membrane ne precoated anti-human IgM antibody, ichiita burgundy colored M band, zvichiratidza W. bancrofti kana B. malayi IgM positive test result.
W. bancrofti kana B. malayi Masoja ekudzivirira chirwere eIgG kana aripo mumuenzaniso anosunga kuFilariasis conjugates.Iyo immunocomplex inobva yatorwa ne-pre-coated reagents pa membrane, ichiita burgundy colored G band, zvichiratidza W. bancrofti kana B. malayi IgG positive test result.
Kusavapo kwechero mabhendi ebvunzo (M naG) kunoratidza mhedzisiro yakaipa.Iyo bvunzo ine yemukati kudzora (C bhendi) iyo inofanirwa kuratidza burgundy ruvara bhendi ye immunocomplex yembudzi anti rabbit IgG/tsuro IgG-goridhe conjugate zvisinei nekusimudzirwa kwemavara pane chero mabhendi ebvunzo.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
