PFUPISO UYE TSANANGURO MUYEdzo
Chlamydia pneumoniae (C. pneumoniae) imhando yebhakitiriya inowanzoitika uye chikonzero chikuru chepneumonia pasi rose.Inenge 50% yevanhu vakuru vane humbowo hwehutachiona hwekare pazera remakore makumi maviri, uye kuberekwazve gare gare muupenyu kwakajairika.Ongororo dzakawanda dzakaratidza kubatana kwakananga pakati peC. pneumoniae utachiona nezvimwe zvirwere zvinozvimba zvakaita seatherosclerosis, acute exacerbations of COPD, uye asima.
Kuongororwa kwehutachiona hweC. pneumoniae kunonetsa nekuda kwekutsanya kwechimiro chechirwere chetachiona, seroprevalence yakakura, uye mukana wekufamba kwenguva pasina zviratidzo.Nzira dzakatangwa dzekuongorora marabhoritari dzinosanganisira kuparadzaniswa kwechipenyu musero tsika, serological assays uye PCR.Microimmunofluorescence bvunzo (MIF), ndiyo yazvino "yegoridhe mwero" yekuongorora serological, asi muyedzo uchiri kushaya kumira uye nehunyanzvi hwekuita.Antibody immunoassays ndiyo inonyanya kushandiswa serology bvunzo uye yekutanga chlamydial utachiona hunoonekwa nemhinduro yeIgM yakawanda mukati memavhiki maviri kusvika mana uye kunonoka IgG neIgA mhinduro mukati memavhiki matanhatu kusvika masere.Nekudaro, mukutapurirazve, IgG uye IgA mazinga anosimuka nekukurumidza, kazhinji mumavhiki e1-2 nepo IgM mazinga asingawanzo kuwanikwa.Nechikonzero ichi, masoja ekudzivirira chirwere eIgA akataridza kuve akavimbika ekudzivirira kwezvirwere zvekutanga, zvisingaperi uye zvinowanzoitika kunyanya kana zvasanganiswa nekuonekwa kweIgM.
PRINCIPLE
Chlamydia pneumoniae IgG/IgM Rapid Test kit inobva pamusimboti we qualitative immunochromatographic assay yekuziva Chlamydia pneumoniae IgG/IgM antibody iri muserum yevanhu, plasma kana ropa rose. e Antigen conjugates), 2) nitrocellulose membrane strip ine test band (T bhendi) uye control bhendi (C band).Iro T bhendi rakafanoputirwa nembeva rinopesana nemunhu IgG antibody, uye C bhendi rakafanoputirwa nembudzi anti-mbeva IgG antibody.Strip B ine: 1) burgundy colored conjugate pad ine C. pneumoniae antigen yakasanganiswa necolloid gold (C. pneumoniae Antigen conjugates), 2) a
nitrocellulose membrane strip ine test band (T bhendi) uye control bhendi (C bhendi).Iro T bhendi rakafanoputirwa nembeva rinopesana nemunhu IgM antibody, uye C bhendi rakafanoputirwa nembudzi anti-mbeva IgG antibody.
Mutsetse A: Kana huwandu hwakakwana hwemuenzaniso webvunzo ukaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito pakaseti.C.pneumoniae IgG masoja ekudzivirira chirwere kana aripo mumuenzaniso anosunga kuC. pneumoniae Antigen conjugates.Iyo immunocomplex inozobatwa pa membrane neiyo pre-yakavharwa Mouse anti-human IgG antibody, ichigadzira burgundy yemavara T bhendi,
zvichiratidza C. pneumoniae IgG positive test result.Kusavapo kweT band kunoratidza mhedzisiro yakaipa.Muedzo une mukati mekutonga (C bhendi) iyo inofanirwa kuratidza burgundy yemavara bhendi ye immunocomplex yembudzi anti-mouse IgG/mouse IgGgold conjugate zvisinei nekuvepo kwemavara T bhendi.Zvikasadaro, bvunzo mhinduro
harisi kushanda uye mufananidzo wacho unofanirwa kuongororwa nemumwe mudziyo.
Strip B: Kana huwandu hwakakwana hwebvunzo hwemuenzaniso hwaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito pakaseti.C.pneumoniae IgM masoja ekudzivirira chirwere kana aripo mumuenzaniso anosunga kuC. pneumoniae Antigen conjugates.Iyo immunocomplex inozobatwa pa membrane neiyo pre-yakavharwa Mouse anti-human IgM antibody, ichigadzira burgundy yemavara T bhendi,
zvichiratidza C. pneumoniae IgM positive test result.Kusavapo kweT band kunoratidza mhedzisiro yakaipa.Muedzo une mukati mekutonga (C bhendi) iyo inofanirwa kuratidza burgundy yemavara bhendi ye immunocomplex yembudzi anti-mouse IgG/mouse IgGgold conjugate zvisinei nekuvepo kwemavara T bhendi.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
