PFUPISO UYE TSANANGURO MUYEdzo
Typhoid fever inokonzerwa neS. typhi, utachiona hweGram-negative.Munyika yose inofungidzirwa mamiriyoni 17 ezviitiko uye 600,000 dzinofa dzakabatanidzwa dzinoitika gore negore.Varwere vane hutachiwana hweHIV vari panjodzi yakawedzerwa zvikuru yekubatwa nechirwere cheS. typhi.Humbowo hwehutachiona hweH. pylori hunopawo njodzi yekuwedzera yekuwana typhoid fever.1-5% yevarwere vanove vatakuri vasingaperi vakachengeta S. typhi mugallbladder.
Kuongororwa kwekliniki yefivha yetyphoid kunoenderana nekuparadzaniswa kweS. typhi kubva muropa, mwongo wemapfupa kana chironda chaicho cheanatomic.Muzvivakwa zvisingakwanise kuita iyi yakaoma uye inopedza nguva maitiro, Filix-Widal bvunzo inoshandiswa kufambisa kuongororwa.Nekudaro, zvipingamupinyi zvakawanda zvinotungamira kumatambudziko mukududzirwa kweiyo Widal bvunzo.
Mukupesana, theTyphoid IgG/IgM Rapid Test Kit iri nyore uye rinokurumidza murabhoritari bvunzo.Muedzo unoona panguva imwe chete uye unosiyanisa IgG neIgM masoja ekudzivirira chirwere kuS. typhi chaiwo antigen mumuenzaniso weropa rose nokudaro rubatsiro mukutsunga kwekusangana kwazvino kana kwekare kune S. typhi.
PRINCIPLE
Iyo Typhoid IgG/IgM Combo Rapid Test is a lateral flow chromatographic
immunoassay.The test cassette ine: 1) burgundy colored conjugate pad ine recombinant S. typhoid H antigen uye O antigen conjugated ne colloid goridhe (Typhoid conjugates) uye tsuro IgG-goridhe conjugates, 2) nitrocellulose membrane strip ine maviri test band (M uye G band).Iyo M bhendi yakafanoputirwa nemonoclonal anti-human IgM yekuonekwa kweIgM anti-S.typhi, G bhendi rakafanoputirwa nemareagents ekuonekwa kweIgG
anti-S.typhi, uye bhendi reC rakafanoputirwa nembudzi anti tsuro IgG.
Kana vhoriyamu yakakwana yebvunzo yemuenzaniso yaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.Anti-S.typhi IgM kana iripo mumuenzaniso inosungirirana neTyphoid conjugates.Iyo immunocomplex inobva yatorwa pa membrane ne-pre-coated anti-human IgM antibody, ichiita burgundy colored M band, zvichiratidza S. typhi IgM positive test result.
Anti-S.typhi IgG kana iripo mumuenzaniso inosungirirana neTyphoid conjugates.Iyo immunocomplex inobva yatorwa ne-pre-coated reagents pa membrane, ichiita burgundy colored G band, zvichiratidza S. typhi IgG positive test result.
Kusavapo kwechero mabhendi ebvunzo (M naG) kunoratidza mhedzisiro yakaipa.Iyo bvunzo ine yemukati kudzora (C bhendi) iyo inofanirwa kuratidza burgundy ruvara bhendi ye immunocomplex yembudzi anti rabbit IgG/tsuro IgG-goridhe conjugate zvisinei nekusimudzirwa kwemavara pane chero mabhendi ebvunzo.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
