Tsanangudzo yakadzama
Funga nezve chero zvigadzirwa zvekwakabva vanhu sehutachiona uye zvibate uchishandisa akajairwa biosafety maitiro.
Plasma
1. Unganidza mufananidzo weropa mu lavender, bhuruu kana girini yepamusoro yekuunganidza chubhu (ine EDTA, citrate kana heparin, maererano muVacutainer®) neveinpuncture.
2.Kuparadzanisa plasma ne centrifugation.
3.Nyatsobvisa plasma muchubhu itsva isati yanyorwa.
Serum
1. Unganidza mufananidzo weropa mutsvuku yepamusoro yekuunganidza chubhu (isina anticoagulants muVacutainer®) neveinpuncture.
2.Rega ropa rivhare.
3.Kuparadzanisa serum ne centrifugation.
4.Nyatsobvisa serum muchubhu itsva yakanyorwa kare.
5.Test specimens nokukurumidza sezvinobvira mushure mekuunganidza.Chengetedza sampuli pa2°C kusvika 8°C kana isina kuongororwa nekukasika.
6.Chengetedza sampuli pa2°C kusvika pa8°C kusvika kumazuva mashanu.Mienzaniso inofanira kuiswa muchando pa -20 ° C kuitira kuchengetedza kwenguva refu
Ropa
Madonhwe eropa rose anogona kuwanikwa nekubaya kwemunwe tip kana kuputika kwetsinga.Usashandise chero ropa rehemolized kuongororwa.Zviyero zveropa rose zvinofanira kuchengetwa mufiriji (2°C-8°C) kana zvisina kuongororwa nekukasika.Mienzaniso inofanira kuongororwa mukati memaawa makumi maviri nemana ekuunganidza.Dzivisa maitiro akawanda echando.Usati waedza, hunza zvimedu zvakaomeswa nechando kune tembiricha yekamuri zvishoma nezvishoma uye sanganisa zvinyoro.Mienzaniso ine zvinhu zvinooneka zvinofanira kujekeswa ne centrifugation isati yaedzwa.
ASAY PROCEDURE
Danho 1: Uya nemuenzaniso uye bvunzo zvikamu kune tembiricha yekamuri kana iri firiji kana chando.Kana yanyungudutswa, sanganisa sampuli zvakanaka usati waedza.
Nhanho yechipiri: Kana wagadzirira kuyedza, vhura homwe pane notch uye bvisa mudziyo.Isa mudziyo wekuyedza panzvimbo yakachena, yakati sandara.
Danho rechitatu: Iva nechokwadi chekuisa chiratidzo pamudziyo wacho nenhamba yeID yemuenzaniso.
Danho rechina: Kuongororwa ropa rose - Isa donhwe rimwe reropa rose (ringaita 30-35 µL) mumucherechedzo mutsime.- Wobva wawedzera madonhwe maviri (angangoita 60-70 µL) eSample Diluent nekukurumidza.