RV IgG Rapid Test Uncut Sheet

RV IgG Rapid Test

Type: Uncut Sheet

Mucherechedzo: Bio-mapper

Catalog:RT0521

Muenzaniso:WB/S/P

Sensitivity: 91.10%

Kunyanya: 99%

Rubella inowanzoitika muvana vezera rechikoro nevachiri kuyaruka, uye vanopfuura 80% yevagari vane hutachiona hwehutachiona uhu.Vakadzi vane pamuviri vakatapukirwa nehutachiona hwerubella vasati vasvika mavhiki makumi maviri ekuzvitakura, uye chiitiko che fetal teratogenesis chakanga chakakwirira.Vakuru nevana vane rubella virus vanogona kukonzera mapundu eganda.ELISA: Mutakuri akaputirwa neinactivated rubella virus antigen anogona kusunga neakananga antibody mumuenzaniso wakaedzwa, uye antibody inoenderana inoonekwa ne enzyme yakanyorwa kuti anti human immunoglobulin uye substrate.

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Tsanangudzo yakadzama

(1) Kuti uunganidze sampuli uye bvunzo yekuongorora, chete sampu yeropa imwe chete inoda kuunganidzwa.Zvisinei, kana zvakakodzera kutonga mamiriro ekudzivirira ezvirwere zvevanhu vane utachiona, zvakakosha kutora sampuli kubva kune vanofungidzirwa kuti vane rubella varwere mukati memazuva matatu mushure mekutanga kwekuputika uye zvinotevera 14 kusvika kumazuva 21 kuti zvionekwe panguva imwe chete.
(2) Zvakafanana neiyo general ELISA, wedzera PBS 50 mugomba rega rega rekutonga uye sampuli μ l.Ramba uchiwedzera muenzaniso 10 μ l.Kupisa pa25 ℃ kwe45 min, geza uye woomesa.
(3) Wedzera maenzayimu mamaki kune yega yega 250 μ l.Nzira imwechete inoshandiswa pakuchengetedza kupisa uye kushambidza.
(4) Wedzera pNPP substrate mhinduro 250 μ l.Mushure mekuchengetedza kupisa uye kugezwa nenzira imwecheteyo, wedzera 1mol / L sodium hydroxide 50 μ L Misa maitiro, kuyera kukosha kwekunyura kwegomba rimwe nerimwe pa405nm, uye kutonga chigumisiro chemuenzaniso wakaedzwa.
(5) Kana iri mhedzisiro yakanaka, sampuli inogona kuwedzeredzwa kuti ione iyo antibody titer, enzanisa mhedzisiro yemasampuli maviri akateedzana, uye mutongi.