Tsanangudzo yakadzama
Kuonekwa kwehwai pox virus antibody inoumbwa ne microplate pre-yakaputirwa nehwai pox nuclear protein antigen, enzyme markers uye mamwe anotsigira reagents, uye musimboti we enzyme-yakabatana immunoassay (ELISA) inoshandiswa kuona iyo makwai pox antibody mune yehwai serum sample.Munguva yekuedza, seramu yekutonga uye sampuli inofanira kuongororwa inowedzerwa kune microplate plate, uye kana sampuli ine makwai pox antibody mushure mekuiswa, inosungirirwa kune antigen pane microplate plate, uye zvimwe zvikamu zvisina kusungwa zvichabviswa mushure mekugeza;Wobva wawedzera enzyme marker kuti unyatsosunga kune antigen-antibody complex pane microplate plate;Iwo ma enzyme marker asina kusungwa akabva abviswa nekugezeswa, uye TMB substrate solution yakawedzerwa kumatsime, uye chigadzirwa chebhuruu chakaumbwa nekuita kweiyo microplate conjugates, uye kudzika kwemavara kwakanyatso kuwirirana nehuwandu hwemasoja ekudzivirira arimo mumuenzaniso.Mushure mokunge mhinduro yekugumisa yakawedzerwa kugumisa maitiro, chigadzirwa chakashanduka chero;Iko kukosha kwekutora mukuita kwega kwega kunotemerwa nemuverengi wemicroplate paavelength ye450 nm kuona kana sampuli ine ma antibodies ehwai pox.