PFUPISO UYE TSANANGURO MUYEdzo
Malaria chirwere chinotakurwa neumhutu, hemolytic, febrile chinobata vanhu vanopfuura mamirioni mazana maviri uye chinouraya vanhu vanopfuura miriyoni imwe pagore.Inokonzerwa nemhando ina dzePlasmodium: P. falciparum, P. vivax, P. ovale, uye P. malariae.Iyi plasmodia yose inotapukira uye inoparadza erythrocytes yevanhu, ichibudisa chills, fever, anemia, uye splenomegaly.P. falciparum inokonzeresa zvirwere zvakanyanya kudarika mamwe marudzi eplasmodial uye inokonzeresa ndufu zhinji dzemalaria.P. falciparum neP. vivax ndiwo utachiona hunozivikanwa zvikuru, zvisinei, pane musiyano mukuru wenzvimbo mukuparadzirwa kwemarudzi.
Sechinyakare, marariya inoonekwa nekuratidzwa kwezvipenyu paGiemsa yakasvibiswa gobvu smears yeropa rekutenderera, uye marudzi akasiyana eplasmodium anosiyaniswa nekuonekwa kwavo mune ane hutachiona erythrocytes1.Iyo tekinoroji inokwanisa kunyatso uye yakavimbika kuongororwa, asi chete kana ichiitwa nehunyanzvi maikorosikopu vachishandisa yakatsanangurwa maprotocol2, ayo anopa zvipingamupinyi zvikuru kunzvimbo dziri kure nedzirombo dzepasirese.
Iyo Malaria Pf / Pan Antigen Rapid Test Kit inogadzirwa kugadzirisa izvi zvipingamupinyi.Muedzo unoshandisa maviri emonoclonal uye polyclonal antibodies kuP. falciparum chaiyo protein, Histidine Repeat Protein II (pHRP-II), uye maviri emonoclonal antibodies kuplasmodium Lactate Dehydrogenase (pLDH), puroteni inogadzirwa nemhando ina dze plasmodium, nokudaro uye inogonesa kuonekwa kwakasiyana kwePlasmodium nemhando dzakasiyana siyana dzepaplasmodium. odia.Inogona kuitwa nevashandi vasina kudzidziswa kana vashoma vane hunyanzvi, vasina midziyo yemurabhoritari.
PRINCIPLE
Iyo Pf/ Pan Malaria Rapid Test kit ndeye lateral inoyerera chromatographic immunoassay.Iwo bvunzo strip zvikamu zvinosanganisira: 1) burgundy colored conjugate pad ine mbeva anti- pHRP-II antibody yakasanganiswa negoridhe colloid (pHRP II-goridhe conjugates) uye mbeva anti-pLDH antibody yakasanganiswa negoridhe colloid (pLDH-goridhe conjugates),
2) nitrocellulose membrane strip ine maviri ebvunzo mabhendi (Pan uye Pv mabhendi) uye control bhendi (C bhendi).Pan band yakagara yakavharwa nemonoclonal anti-pLDH antibody iyo iyo hutachiona hwemhando ipi neipi yemhando ina dzeplasmodia inogona kuonekwa, bhendi rePf rakafanoputirwa nepolyclonal anti-pHRP-II masoja ekudzivirira ekudzivirira chirwere chePf, uye C band yakaputirwa nembudzi anti-mouse IgG.
Munguva yekuedzwa, huwandu hwakakwana hwemuenzaniso weropa unopihwa mumuenzaniso tsime (S) yekaseti yekuedzwa, lysis buffer inowedzerwa kune buffer tsime (B).Iyo buffer ine detergent inoisa masero matsvuku eropa uye inoburitsa akasiyana plasmodium antigens, ayo anofamba necapillary chiito kuyambuka mutsara wakabatwa mukaseti.pHRP-II kana zvipo mumuenzaniso zvichasunga kune pHRP II-goridhe conjugates.Iyo immunocomplex inozobatwa pa membrane ne pre-coated anti-pHRPII masoja ekudzivirira chirwere, achigadzira burgundy ine ruvara rwePf bhendi, zvichiratidza mhedzisiro yePf yakanaka.pLDH kana zvipo mumuenzaniso zvinosungirira kune pLDH goridhe conjugates.Iyo immunocomplex inozobatwa pa membrane ne pre-coated anti pLDH antibody, ichigadzira burgundy yemavara Pan bhendi, ichiratidza plasmodium yakanaka bvunzo mhedzisiro.Mukushaikwa kwePan bhendi, mhedzisiro yebvunzo yakanaka kune chero yemamwe matatu plasmodia inogona kukurudzirwa.
Kusavapo kwechero mabhendi ebvunzo (Pan nePf) zvinoratidza mhedzisiro yakaipa.Iyo bvunzo ine yemukati kudzora (C bhendi) iyo inofanirwa kuratidza burgundy ruvara bhendi ye immunocomplex yembudzi anti-mbeva IgG / mbeva IgG (pHRP-II uye pLDH-goridhe conjugates) zvisinei nekukura kwemavara pane chero mabhendi ekuedzwa.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
