PFUPISO UYE TSANANGURO MUYEdzo
Malaria chirwere chinotakurwa neumhutu, hemolytic, febrile chinobata vanhu vanopfuura mamirioni mazana maviri uye chinouraya vanhu vanopfuura miriyoni imwe pagore.Inokonzerwa nemhando ina dzePlasmodium: P. falciparum, P. vivax, P. ovale, uye P. malariae.Iyi plasmodia yose inotapukira uye inoparadza erythrocytes yevanhu, ichibudisa chills, fever, anemia, uye splenomegaly.P. falciparum inokonzera zvirwere zvakakomba kupfuura mamwe marudzi eplasmodial uye inokonzera nzufu dzakawanda dzemarariya, uye ndeimwe yezvirwere zviviri zvinonyanya kuzivikanwa zvemarariya.
Sechinyakare, marariya inowonekwa nekuratidzwa kwezvipenyu paGiemsa yakasvibiswa smears yeropa reperipheral, uye marudzi akasiyana eplasmodium anosiyaniswa nekuonekwa kwavo mune ane hutachiona erythrocytes.Iyo tekinoroji inokwanisa kunyatso uye yakavimbika kuongororwa, asi chete kana ichiitwa nehunyanzvi maikorosikopu vachishandisa yakatsanangurwa maprotocol, ayo anopa zvipingamupinyi zvikuru kunzvimbo dziri kure nedzirombo dzepasirese.
Iyo Pf Ag Rapid Test inogadzirwa kugadzirisa izvi zvipingamupinyi.Iyo inoona iyo Pf chaiyo antigen pHRP-II muropa remunhu muenzaniso.Inogona kuitwa nevashandi vasina kudzidziswa kana vashoma vane hunyanzvi, vasina midziyo yemurabhoritari.
PRINCIPLE
Iyo Pf Ag Rapid Muedzo ndeye lateral kuyerera chromatographic immunoassay.Iwo bvunzo strip zvikamu zvinosanganisira: 1) burgundy colored conjugate pad ine monoclonal anti- pHRP-II antibody yakasanganiswa negoridhe colloid (pHRP II-goridhe conjugates), 2) nitrocellulose membrane strip ine test bhendi (Pf) uye control bhendi (C bhendi).Iyo Pf bhendi yakagara yakavharwa nepolyclonal anti-pHRP-II masoja ekudzivirira chirwere, uye C bhendi rakafambwa nembudzi anti-mbeva IgG.
Munguva yekuedzwa, huwandu hwakakwana hwemuenzaniso weropa unopihwa mumuenzaniso tsime (S) yekaseti yekuedzwa, lysis buffer inowedzerwa kune buffer tsime (B).Iyo buffer ine detergent iyo inoisa masero matsvuku eropa uye inoburitsa pHRPII antigen, iyo inofamba necapillary chiito ichiyambuka mutsara wakabatwa mukaseti.pHRP-II kana zvipo mumuenzaniso zvichasunga kune pHRP II-goridhe conjugates.Iyo immunocomplex inozobatwa pa membrane ne pre-coated polyclonal antipHRP II masoja ekudzivirira chirwere, achigadzira burgundy colored Pf bhendi, zvichiratidza mhedzisiro yePf yakanaka.
Kusavapo kwePf bhendi kunoratidza mhedzisiro yakaipa.Iyo bvunzo ine yemukati kudzora (C bhendi) iyo inofanirwa kuratidza burgundy ruvara bhendi ye immunocomplex yembudzi anti-mbeva IgG / mbeva IgG (pHRP II-goridhe conjugates) zvisinei nekukura kwemavara pane chero Pf bhendi.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
