PFUPISO UYE TSANANGURO MUYEdzo
Visceral leishmaniasis, kana Kala-azar, chirwere chinoparadzirwa chinokonzerwa nemhando dzakawanda dzeL. donovani.Chirwere ichi chinofungidzirwa neWorld Health Organisation (WHO) kuti chabata vanhu vanosvika mamiriyoni gumi nemaviri munyika makumi masere nemasere.Inotapuriranwa kuvanhu nekurumwa kwePhlebotomus sandflies, iyo inowana hutachiona kubva mukudyisa mhuka dzine hutachiona.Kunyange zvazvo chiri chirwere chinowanikwa munyika dzine urombo, kuSouthern Europe, ndicho chazova chirwere chinotapukira chinotapukira muvarwere veAIDS.Kuzivikanwa kweL. donovani organism kubva muropa, bone marrow, chiropa, lymph nodes kana spleen inopa nzira chaiyo yekuongororwa.Serological kuonekwa kweAnti-L.donovani IgM inowanikwa kuva yakanakisa mucherechedzo weacute Visceral leishmaniasis.Miedzo inoshandiswa mukiriniki inosanganisirwa ELISA, fluorescent antibody kana yakananga agglutination bvunzo 4-5.Munguva pfupi yapfuura, kushandiswa kweL. donovani chaiyo mapuroteni muyedzo kwakavandudza kunzwisiswa uye hunhu zvakanyanya.
Iyo Leishmania IgG/IgM Combo Rapid Test ndeye recombinant protein based serological test, iyo inoona masoja ekudzivirira chirwere eIgG neIgM kuL. Donovani panguva imwe chete.Muedzo unopa mhedzisiro yakavimbika mukati memaminitsi gumi nemashanu pasina zviridzwa.
PRINCIPLE
Iyo Leishmania IgG/IgM Rapid Test is a lateral flow chromatographic immunoassay.The test cassette ine: 1) burgundy colored conjugate pad ine recombinant L. donovani antigen conjugated ne colloid gold (Leishmania conjugates) uye tsuro IgG-goridhe conjugates, 2) nitrocellulose membrane strip ine maviri ebvunzo mabhendi (T1 uye T2 mabhendi) uye bhandi rekutonga (C band).Iro T1 bhendi rakafanoputirwa nemonoclonal anti-human IgM yekuona anti-L.donovani IgM, T2 bhendi yakavharwa nemareagents ekuona anti-L.donovani IgG, uye C bhendi yakashongedzwa nembudzi anti tsuro IgG.
Kana vhoriyamu yakakwana yemuenzaniso webvunzo yaiswa mumuenzaniso tsime rekaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.Iyo L. donovani IgM kana iripo mumuenzaniso inosungirira kune Leishmania conjugates.Iyo immunocomplex inobva yatorwa pa membrane ne pre-coated anti-human IgM antibody, ichiita burgundy colored T1 bhendi, zvichiratidza L. donovani IgM positive test result.Iyo L. donovani IgG kana iripo mumuenzaniso inosungirira kune Leishmania conjugates.Iyo immunocomplex inobva yatorwa ne-pre-coated reagents pa membrane, ichiita burgundy colored T2 band, zvichiratidza L. donovani IgG positive test result.
Kusavapo kwechero T mabhendi (T1 neT2) inoratidza mhedzisiro yakaipa.Iyo bvunzo ine yemukati kudzora (C bhendi) iyo inofanirwa kuratidza burgundy yemavara bhendi ye immunocomplex yembudzi anti rabbit IgG/tsuro IgG-goridhe conjugate zvisinei nekukura kwemavara pane chero maT bhendi.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
