HIV (I+II) Antibody Test (mitsetse miviri)

HIV (I+II) Antibody Test (mitsetse miviri)

Type: Uncut Sheet

Mucherechedzo: Bio-mapper

Catalog:RF0171

Muenzaniso: Weti

Utachiona hweAIDS, hunozivikanwawo sehumwe hutachiwana hwehutachiona hweHIV (HIV), hutachiona hunogona kurwisa T4 lymphocytes, chikamu chakakosha chemuviri wemunhu.Masoja ekudzivirira eHIV (HIV AB) ari muropa revanhu vane HIV, zvisinei nekuti vane zviratidzo kana kuti vasina.Nokudaro, kuonekwa kweHIV AB chiratidzo chakakosha chekuonekwa kwehutachiwana hweHIV.Kuti uone kuti munhu ane HIV here, nzira yakajairika yekuongorora ndeyekuenda kuzvipatara kunoongororwa masoja emuropa.Muyero weHIV Ab test ndeye serum antibody test.Pane nzira dzakawanda dzekuongorora HIV Ab kumba nekune dzimwe nyika, dzinogona kukamurwa kuita enzyme-yakabatana immunosorbent assay, agglutination assay uye immunochromatography maererano nemisimboti yekuona dzakasiyana.Mubasa rinoshanda, enzyme yakabatana immunosorbent assay, gelatin agglutination bvunzo uye akasiyana anokurumidza diagnostic reagents anowanzoshandiswa.

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Tsanangudzo yakadzama

Western blot (WB), strip immunoassay (LIATEK HIV Ⅲ), radioimmunoprecipitation assay (RIPA) uye immunofluorescence assay (IFA).Iyo inowanzo shandiswa yekusimbisa bvunzo nzira muChina ndeye WB.

(1) Western blot (WB) inzira yekuyedza inoshandiswa zvakanyanya mukuongororwa kwezvirwere zvakawanda zvinotapukira.Nezvekuongororwa kwehutachiona hweHIV, ndiyo yekutanga nzira yekusimbisa yekuyedza inoshandiswa kusimbisa masoja ekudzivirira eHIV.Mhedzisiro yekuonekwa kweWB inowanzo shandiswa se "goridhe mwero" kuona zvakanakira nekuipira kwedzimwe nzira dzekuyedza.
Simbiso yekuedza maitiro:
Kune HIV-1/2 yakasanganiswa mhando uye imwe chete HIV-1 kana HIV-2 mhando.Kutanga, shandisa HIV-1/2 yakasanganiswa reagent kuti uongorore.Kana mhinduro isiri iyo, taura kuti masoja ekudzivirira eHIV haana;Kana iri positive, inozivisa kuti iHIV-1 antibody positive;Kana maitiro akanaka akasazadzikiswa, zvinotongwa kuti mhedzisiro yeHIV antibody haina chokwadi.Kana paine bhendi rechiratidzo cheHIV-2, unofanira kushandisa HIV-2 immunoblotting reagent kuita HIV 2 masoja ekudzivirira chirwere zvakare, izvo zvinoratidza kusaita zvakanaka, uye taura kuti masoja ekudzivirira eHIV 2 haana;Kana iine positive, inozotaura kuti iine serologically yeHIV-2 masoja ekudzivirira chirwere, uye yotumira sample kuNational reference laboratory for nucleic acid sequence analysis.
Kunzwa kweWB kazhinji hakuna kuderera pane iyo yekutanga yekuongorora bvunzo, asi iyo chaiyo yakakwira zvakanyanya.Izvi zvinonyanya kubva pakuparadzaniswa, kusungirirwa uye kucheneswa kwezvikamu zvakasiyana-siyana zveHIV antigen, izvo zvinogona kuona masoja ekudzivirira zvirwere zvakasiyana-siyana, saka nzira yeWB inogona kushandiswa kuona chokwadi chekuongorora kwekutanga.Inogona kuonekwa kubva kuWB yekusimbisa migumisiro yekuedza kuti kunyange zvazvo ma reagents ane unhu hwakanaka anosarudzwa kuti aonekwe kwekutanga kuongorora, zvakadai sechizvarwa chechitatu ELISA, pachange paine zviratidzo zvenhema, uye migumisiro yakarurama inogona kuwanikwa chete kuburikidza nemuedzo wekusimbisa.
(2) Immunofluorescence assay (IFA)
IFA nzira ine hupfumi, yakapusa uye inokurumidza, uye yakakurudzirwa neFDA kuongororwa kweWB isina chokwadi samples.Nekudaro, mamicroscopes anodhura efluorescent anodiwa, nyanzvi dzakanyatsodzidziswa dzinodiwa, uye mhedzisiro yekutarisa uye yekududzira inokanganiswa zviri nyore nezvinhu zvinomiririra.Mhedzisiro yacho haifanirwe kuchengetedzwa kwenguva yakareba, uye IFA haifanirwe kuitwa uye kuiswa mumarabhoritari akajairwa.
Mushumo wemhinduro dzebvunzo dzeHIV antibody
Mhedzisiro yeongororo yekusimbisa masoja ekudzivirira chirwere chemukondombera ichataurwa muAttached Table 3.
(1) Tevedzera maitiro ekutonga ane HIV 1 masoja ekudzivirira chirwere, taura kuti “HIV 1 antibody positive (+)”, uye ita basa rakanaka rekubvunzana mushure mekuongororwa, kuvanzika uye mamiriro edenda sezvinodiwa.Tevedzera maitiro ekutonga ane HIV 2 masoja ekudzivirira chirwere, taura "HIV 2 antibody positive (+)", uye ita basa rakanaka rekubvunzana mushure mekuongororwa, kuvanzika uye mamiriro edenda sezvinodiwa.
(2) Ita zvinoenderana nemaitiro ekutonga ehutachiona hweHIV, uye taura "HIV antibody negative (-)".Muchiitiko chekufungidzirwa kwehutachiona hwe "window period", kumwezve kuongororwa kweHIV nucleic acid kunokurudzirwa kuita chirwere chakajeka nekukurumidza.
(3) Ita zvinoenderana nemaitiro ekusava nechokwadi kwemasoja eHIV, taura "kusava nechokwadi kwemasoja eHIV (±)", uye ona mumashoko anoti "kumirira kuongororwazve mushure memavhiki mana".