PFUPISO UYE TSANANGURO MUYEdzo
Lymphatic filariasis inozivikanwa seElephantiasis, inonyanya kukonzerwa neW. bancrofti naB. malayi, inobata vanhu vanenge mamiriyoni 120 munyika dzinopfuura 80.Chirwere ichi chinotapuriranwa kuvanhu nekurumwa neumhutu hune utachiona mukati umo microflariae inoyamwa kubva kune ine hutachiona hwemunhu inokura kuita nhanho yetatu.Kazhinji, kudzokorora uye kwenguva refu kuratidzwa kune utachiona hwemakonye kunodiwa pakugadzwa kwehutachiona hwevanhu.
The definitive parasitologic diagnosis ndiyo kuratidzwa kwe microflariae mumasampuli eropa.Nekudaro, iyi chiyero chegoridhe bvunzo inoganhurirwa nezvinodiwa zvekuunganidza ropa kwehusiku uye kushaikwa kwekunzwa kwakakwana.Kuonekwa kwemaantigen anotenderera kunowanikwa mune zvekutengesa.Kubatsira kwayo kunogumira kuW. bancrofti.Mukuwedzera, microfilaremia uye antigenemia inokura kubva pamwedzi kusvika kumakore mushure mekuratidzwa.
Kuonekwa kwemasoja ekudzivirira chirwere kunopa nzira yekutanga kuona filarial parasite utachiona.Kuvapo kweIgM kune maantigen eparasite kunoratidza hutachiona hwazvino, nepo, IgG inoenderana nedanho rekupedzisira rehutachiona kana hutachiona hwekare.Uyezve, kuzivikanwa kwemaantigen akachengetedzwa kunobvumira 'pan-filaria' bvunzo kuti ishande.Kushandisa recombinant mapuroteni kunobvisa kusangana nevanhu vane zvimwe zvirwere zveparasitic.
Filariasis Ab Rapid Test inoshandisa maantigen akachengetwa kuti aone Antibody panguva imwe chete kuW. bancrofti neB. malayi parasite pasina kurambidzwa kuunganidzwa kwemuenzaniso.
PRINCIPLE
The Filariasis Ab Rapid Test is a lateral flow chromatographic immunoassay.Iyo bvunzo kaseti ine: 1) burgundy yeruvara conjugate pad ine recombinant Filariasis chaiyo antigen yakasanganiswa negoridhe colloid (Filariasis conjugates) uye tsuro IgG-goridhe conjugates, 2) nitrocellulose membrane strip ine test bhendi (T bhendi) uye yekudzora. bhendi (C bhendi).T bhendi rakafanoputirwa neun-conjugated Filariasis antigen, uye C bhendi rakafanoputirwa nembudzi anti-tsuro IgG antibody.
Kana vhoriyamu yakakwana yebvunzo yemuenzaniso yaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.AntiFilariasis Ab kana iripo mumuenzaniso inosunga kune Filariasis conjugates.Iyo immunocomplex inozotorwa pa membrane ne pre-coated antigen, ichigadzira burgundy colored T band, zvichiratidza aFilariasis Ab positive test result.Kusavapo kweT band kunoratidza mhedzisiro yakaipa.Muedzo une mukati mekutonga (C bhendi) iyo inofanirwa kuratidza burgundy yemavara bhendi ye immunocomplex yembudzi anti-tsuro IgG/tsuro IgG-goridhe conjugate zvisinei nekusimudzirwa kwemavara paT band.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
