PFUPISO UYE TSANANGURO MUYEdzo
Mavhairasi eDengue, mhuri ye4 akasiyana serotypes evhairasi (Den 1,2,3,4), ine singlestrained, yakafukidzwa, yakanaka-pfungwa RNA mavhairasi.Hutachiona uhu hunotapurirwa nehumhutu hwemhuri yeStegemyia inoruma masikati, kunyanya Aedes aegypti, uye Aedes albopictus.Nhasi, vanhu vanopfuura mabhiriyoni 2,5 vanogara munzvimbo dzinopisa dzeAsia, Africa, Australia, uye America vari pangozi yokubatwa nechirwere chedengue.Zvinofungidzirwa kuti mamiriyoni 100 echirwere chedengue fever uye zviitiko 250 000 zvechirwere chedengue chinopisa upenyu chinotyisidzira zvinoitika gore negore pasi rose.
Kuonekwa kweSerological yeIgM antibody ndiyo nzira yakajairika yekuongororwa kwehutachiona hwe dengue.Munguva pfupi yapfuura, kuonekwa kwemaantigen akaburitswa panguva yekudzokorora kwehutachiona mumurwere ane hutachiona kwakaratidza mhedzisiro inovimbisa.Inogonesa kuongororwa kubva pazuva rekutanga mushure mekutanga kwefivha kusvika kuzuva 9, kana chikamu chechipatara chechirwere chapera, saka inobvumira kurapwa kwekutanga kuiswa nokukurumidza4-.Dengue NS1 Rapid Test inogadzirwa kuti ione kutenderera dengue antigen muserum. ,plasma kana ropa rose.Muedzo unogona kuitwa nevashandi vasina kudzidziswa kana hunyanzvi hushoma, vasina michina yemurabhoritari.
PRINCIPLE
Dengue NS1 Rapid Test is a lateral flow chromatographic immunoassay.Iyo bvunzo kaseti ine: 1) burgundy yeruvara conjugate pad ine mbeva anti-dengue NS1 antigen yakasanganiswa negoridhe colloid (Dengue Ab conjugates), 2) nitrocellulose membrane strip ine test bhendi (T bhendi) uye control bhendi (C. bhendi).Iro T bhendi rakafanoputirwa nembeva anti-dengue NS1 antigen, uye C band
yakafanoputirwa nembudzi anti-mouse IgG antibody.Masoja ekudzivirira chirwere edengue anocherechedza maantigen kubva muzvikamu zvina zveutachiona hwedengue.
Kana vhoriyamu yakakwana yemuenzaniso webvunzo yaiswa mumuenzaniso tsime rekaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti yebvunzo.Dengue NS1 Ag kana iripo mumuenzaniso inosunga kune Dengue Ab conjugates.Iyo immunocomplex inozobatwa pa membrane neiyo pre-yakavharwa mbeva antiNS1 antibody, ichigadzira burgundy yeruvara T bhendi, inoratidza Dengue Ag yakanaka bvunzo mhedzisiro.
Kusavapo kweT band kunoratidza mhedzisiro yakaipa.Muedzo une mukati mekutonga (C bhendi) iyo inofanirwa kuratidza burgundy yemavara bhendi ye immunocomplex yembudzi anti-mouse IgG/mouse IgG-goridhe conjugate zvisinei nekuvepo kwemavara T bhendi.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
