PFUPISO UYE TSANANGURO MUYEdzo
Mavhairasi eDengue, mhuri ye4 akasiyana serotypes evhairasi (Den 1,2,3,4), ine singlestrained, yakafukidzwa, yakanaka-pfungwa RNA mavhairasi.Hutachiona uhu hunotapurirwa nehumhutu hwemhuri yeStegemyia inoruma masikati, kunyanya Aedes aegypti, uye Aedes albopictus.Nhasi, vanhu vanopfuura mabhiriyoni 2,5 vanogara munzvimbo dzinopisa dzeAsia, Africa, Australia, uye America vari pangozi yokubatwa nechirwere chedengue.Zvinofungidzirwa kuti mamiriyoni zana echirwere chedengue fever uye 250,000 zviitiko zvinotyisidzira upenyu dengue hemorrhagic fever zvinoitika gore negore pasi rose1-3.
Kuonekwa kweSerological yeIgM antibody ndiyo nzira yakajairika yekuongororwa kwehutachiona hwe dengue.Munguva pfupi yapfuura, kuonekwa kwemaantigen akaburitswa panguva yekudzokorora kwehutachiona mumurwere ane hutachiona kwakaratidza mhedzisiro inovimbisa.Inogonesa kuongororwa kubva pazuva rekutanga mushure mekutanga kwefivha kusvika pazuva re9, kana chikamu chechipatara chechirwere chacho chapera, saka inobvumira kurapwa kwekutanga kwakaiswa nokukurumidza4-.Dengue IgG / IgM Rapid Test inogadzirwa kuti ione kupararira kwe dengue antigen mu serum, plasma kana ropa rose.Muedzo unogona kuitwa nevashandi vasina kudzidziswa kana hunyanzvi hushoma, vasina michina yemurabhoritari.
PRINCIPLE
Dengue IgG/IgM Rapid Test is a lateral flow chromatographic immunoassay.Iyo test cassette ine: 1) burgundy colored conjugate pad ine dengue recombinant envelope antigens yakasanganiswa negoridhe colloid (dengue conjugates) uye tsuro IgG-goridhe conjugates,2) nitrocellulose membrane strip ine maviri ebvunzo mabhendi (G uye M band bhendi) uye bhendi rekutonga.G bhendi rakafambwa nemasoja ekudzivirira chirwere chekudzivirira dengue, M band rakaiswa masoja ekudzivirira chirwere cheIgM anti-dengue virus, uye C band rakafambwa nembudzi anti rabbit IgG.
Kana vhoriyamu yakakwana yebvunzo yemuenzaniso yaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.IgG anti-dengue virus kana iripo mumuenzaniso inosunga kune dengue conjugates.Iyo immunocomplex inozobatwa neiyo reagent yakavharwa paG bhendi, ichigadzira burgundy yemavara G bhendi, ichiratidza hutachiona hwedengue IgG yakanaka bvunzo mhedzisiro uye ichiratidza hutachiona huchangobva kuitika kana kudzokorora.IgM anti-dengue virus, kana iripo mumuenzaniso, inosunga kune dengue conjugates.Iyo immunocomplex inozobatwa neiyo reagent pre-yakavharwa paM bhendi, ichigadzira burgundy ine ruvara rweM bhendi, ichiratidza hutachiona hwedengue IgM positive bvunzo mhedzisiro uye kuratidza hutachiona hutsva.
Kusavapo kwechero mabhendi ekuedzwa (G uye M) anoratidza mhedzisiro yakaipa.Muedzo une mukati mekutonga (C band) iyo inofanirwa kuratidza burgundy colored band ye immunocomplex yembudzi anti rabbit IgG/rabbit IgG-gold conjugate zvisinei nekusimudzirwa kwemavara pane chero maT bands.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
