PFUPISO UYE TSANANGURO MUYEdzo
Cholera chirwere chinotapukira chinotapukira chinoonekwa nekurasikirwa kukuru kwemvura yemumuviri nemagetsi emagetsi kuburikidza nemanyoka akanyanya.Iyo etiological agent yekorera inonzi Vibrio cholerea (V. Cholerae), utachiona hwe gram-negative, hunowanzotapurirwa kuvanhu kuburikidza nemvura ine utachiona uye chikafu.
Mhando yeV. Cholerae yakakamurwa kuva serogroups akati wandei pahwaro hweO antigens.Mapoka madiki O1 neO139 anonyanya kufarira nekuti ese ari maviri anogona kukonzera denda nedenda rekorera.Zvakakosha kuziva nekukurumidza sezvinobvira kuvapo kweV. cholerae O1 neO139 mumakiriniki emuenzaniso, mvura, uye zvokudya kuitira kuti kutarisa kwakakodzera uye maitiro ekudzivirira anobudirira anogona kuitwa nevakuru vehutano hwehutano.
Iyo Cholera Ag Rapid Test inogona kushandiswa zvakananga mumunda nevashandi vasina kudzidziswa kana zvishoma hunyanzvi uye mhedzisiro inowanikwa isingasviki gumi maminetsi, isina inorema murabhoritari michina.
PRINCIPLE
Iyo Cholera Ag Rapid Muedzo ndeye lateral kuyerera chromatographic immunoassay.Iyo test cassette ine: 1) burgundy colored conjugate pad ine monoclonal anti-V.Cholera O1 uye O139 masoja ekudzivirira chirwere akasanganiswa negoridhe colloid (O1/O139-antibody conjugates) uye tsuro IgG-goridhe conjugates, 2) nitrocellulose membrane strip ine maviri ebvunzo bhendi (1 uye 139 bhendi) uye control bhendi (C bhendi).Iro 1 bhendi rakafanoputirwa ne monoclonal anti-V.Cholera O1 antibody.Iyo 139 bhendi yakashongedzwa nemonoclonal anti-V.Cholera O139 antibody.Iyo C bhendi yakafanoputirwa nembudzi anti-mbeva IgG antibody.
Kana vhoriyamu yakakwana yebvunzo yemuenzaniso ikaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.V. Cholera O1/O139 antigen kana iripo mumuenzaniso inosungirira kune inoenderana O1/O139-antibody gold conjugate.Iyi immunocomplex inozotorwa pa membrane ne-pre-coated anti-V.Cholera O1/O139 antibody, ichigadzira burgundy colored test band, zvichiratidza mhedzisiro yeCholera O1/O139.Kusavapo kwebhendi rekuedza kunoratidza mhedzisiro yakaipa.
Muedzo une mukati mekutonga (C bhendi) iyo inofanirwa kuratidza burgundy ruvara bhendi ye immunocomplex yembudzi anti-mouse IgG/ mbeva IgG-goridhe conjugate zvisinei nekusimudzirwa kwemavara pabhendi rekuyedza.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
