PFUPISO UYE TSANANGURO MUYEdzo
Chikungunya chirwere chisingawanzo hutachiona chinotapurirwa nekurumwa nemosquito yeAedes aegypti.Inoratidzwa nekupunduka, kupisa muviri, uye kurwadziwa kwakanyanya kwemajoini (arthralgias) iyo inowanzogara kwemazuva matatu kusvika manomwe.Iri zita rinobva paizwi reMakonde rinoreva kuti "chinokotama" zvichireva kudzikama kwakagadzirwa nekuda kwezviratidzo zvechirwere cheArthritic.Zvinoitika munguva yekunaya kwemvura
munzvimbo dzinopisa dzepasirese, kunyanya muAfrica, South-East Asia, kumaodzanyemba kweIndia nePakistan.Zviratidzo zvinowanzosazivikanwa nekliniki izvo zvinoonekwa mudengue fever.Chokwadi, hutachiona hwe dengue uye chikungunya hwakataurwa muIndia.Kusiyana nedengue, zviratidzo zvekubuda ropa hazviwanzoitiki uye kazhinji hosha iyi ihosha inodzikamisa febrile.Nokudaro zvakakosha zvikuru
kuti vasiyanise dengue kubva kuCHIK chirwere.CHIK inoongororwa yakavakirwa pakuongorora serological uye kuparadzaniswa kwehutachiona mumakonzo kana tsika yematishu.IgM immunoassay ndiyo inonyanya kushanda yebvunzo nzira.Chikungunya IgG/IgM Rapid Test inoshandisa recombinant antigens inotorwa kubva muprotein yayo, inoona IgG/IgM anti-CHIK muserum yemurwere kana plasma mukati memaminitsi makumi maviri.Muedzo unogona kuitwa nevasina kudzidziswa kana
vashandi vane hunyanzvi hushoma, vasina midziyo yemurabhoritari inorema.
PRINCIPLE
Iyo Chikungunya IgG/IgM+NS1 antigen Rapid Test is a lateral flow chromatographic immunoassay.Iyo test cassette ine IgG/IgM Strip uye NS1 strip.
IgG/IgM strip:1) burgundy colored conjugate pad ine Chikungunya recombinant envelope antigens akasanganiswa negoridhe colloid (dengue conjugates) uye tsuro IgG-goridhe conjugates,2) nitrocellulose membrane strip ine maviri ebvunzo mabhendi (G uye M mabhendi) uye a control band (C band).G band rakaiswa kare nemasoja ekudzivirira chirwere cheIgG anti-Chikungunya virus, M band rakaiswa masoja ekudzivirira chirwere cheIgM anti-Chikungunya virus, uye C band rakaiswa kare nembudzi anti-tsuro IgG.
NS1 strip:1) burgundy colored conjugate pad ine mouse anti-Chikungunya NS1 antigen yakasanganiswa necolloid gold (Chikungunya Ab conjugates), 2) nitrocellulose membrane strip ine test band (T bhendi) uye control band (C band).T bhendi rakafanoputirwa netsuro anti-Chikungunya NS1 antigen, uye C band rakafambwa nembudzi anti-mouse IgG antibody.
IgG/IgM strip: Kana huwandu hwakakwana hweyedzo yemuenzaniso hwaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito pakaseti.IgG anti-Chikungunya virus kana iripo mumuenzaniso inosunga kune Chikungunya conjugates.Iyo immunocomplex inozobatwa neiyo reagent yakavharwa paG bhendi, ichigadzira burgundy yeruvara G bhendi, ichiratidza Chikungunya virus IgG yakanaka bvunzo mhedzisiro uye ichiratidza hutachiona huchangobva kuitika kana kudzokorora.IgM anti-Chikungunya virus, kana iripo mumuenzaniso, inosunga kune Chikungunya conjugates.Iyo immunocomplex inozobatwa neiyo reagent pre-yakavharwa paM bhendi, ichigadzira burgundy ine ruvara rweM bhendi, ichiratidza Chikungunya virus IgM positive bvunzo mhedzisiro uye ichiratidza hutachiona hutsva.Kusavapo kwechero mabhendi ekuedzwa (G naM) kunoratidza mhedzisiro yakaipa.Muedzo une control yemukati (C band) iyo inofanirwa kuratidza burgundy colored band ye immunocomplex yembudzi anti rabbit IgG/
tsuro IgG-goridhe conjugate zvisinei nekukura kwemavara pane chero maT band.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
NS1 Strip: Kana vhoriyamu yakakwana yemuenzaniso webvunzo ikaiswa mumuenzaniso tsime rekaseti, mufananidzo wacho unotama necapillary chiito pakaseti yekuedzwa.Chikungunya NS1 Ag kana iripo mumuenzaniso inosunga kune Chikungunya Ab conjugates.Iyo immunocomplex inozobatwa pa membrane neiyo precoated tsuro anti-NS1 antibody, ichigadzira burgundy yeruvara T bhendi, ichiratidza Chikungunya Ag positive test mhedzisiro.Kusavapo kweT band kunoratidza mhedzisiro yakaipa.Muedzo une mukati mekutonga (C bhendi) iyo inofanirwa kuratidza burgundy yemavara bhendi ye immunocomplex yembudzi anti-mouse IgG/mouse IgG-goridhe conjugate zvisinei nekuvepo kwemavara T bhendi.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
