PFUPISO UYE TSANANGURO MUYEdzo
Chikungunya chirwere chisingawanzo hutachiona chinotapurirwa nekurumwa nemosquito yeAedes aegypti.Inoratidzwa nekupunduka, kupisa muviri, uye kurwadziwa kwakanyanya kwemajoini (arthralgias) iyo inowanzogara kwemazuva matatu kusvika manomwe.Iri zita rinobva paizwi reMakonde rinoreva kuti "chinokotama" zvichireva kukombama kwakagadzirwa nekuda kwezviratidzo zvechirwere cheArthritic.Inoitika munguva yekunaya kwemvura munzvimbo dzinopisa dzepasi, kunyanya muAfrica, South-East Asia, kumaodzanyemba kweIndia nePakistan.Zviratidzo zvinowanzosazivikanwa nekliniki izvo zvinoonekwa mudengue fever.Chokwadi, hutachiona hwe dengue uye chikungunya hwakataurwa muIndia.Kusiyana nedengue, zviratidzo zvekubuda ropa hazviwanzoitiki uye kazhinji hosha iyi ihosha inodzikamisa febrile.Saka zvakakosha kusiyanisa dengue kubva kuCHIK chirwere.CHIK inoongororwa yakavakirwa pakuongorora serological uye kuparadzaniswa kwehutachiona mumakonzo kana tsika yematishu.IgM immunoassay ndiyo inonyanya kushanda yebvunzo nzira.Iyo Chikungunya IgG/IgM Rapid Test inoshandisa recombinant antigens inotorwa kubva muchimiro cheprotein, inoona IgG/IgM anti-CHIK mune murwere serum kana plasma mukati memaminitsi makumi maviri.Muedzo unogona kuitwa nevasina kudzidziswa kana
vashandi vane hunyanzvi hushoma, vasina midziyo yemurabhoritari inorema.
PRINCIPLE
Chikungunya IgG/IgM Rapid Test is a lateral flow chromatographic immunoassay.The test cassette ine: 1) burgundy colored conjugate pad ine Chikungunya recombinant envelope antigens conjugated ne colloid gold (dengue conjugates) uye tsuro IgG-goridhe conjugates,2) nitrocellulose membrane strip ine maviri ebvunzo mabhendi (G uye M band bhendi) uye bhendi rekutonga.G band rakaiswa kare nemasoja ekudzivirira chirwere cheIgG anti-Chikungunya virus, M band rakaiswa masoja ekudzivirira chirwere cheIgM anti-Chikungunya virus, uye C band rakaiswa kare nembudzi anti-tsuro IgG.
Kana vhoriyamu yakakwana yebvunzo yemuenzaniso yaiswa mumuenzaniso tsime rebvunzo kaseti, mufananidzo wacho unotama necapillary chiito kuyambuka kaseti.IgG anti-Chikungunya virus kana iripo mumuenzaniso inosunga kune Chikungunya conjugates.Zvigadziriso zvenhamba dzakasiyana-siyana hazvigoni kushandiswa zvakasiyana.Immunocomplex inobva yatorwa neiyo reagent yakavharidzirwa paG band, ichiita burgundy colored G band, ichiratidza Chikungunya virus IgG positive test result uye ichiratidza chirwere chechangobva kuitika kana kudzokorora.IgM anti-Chikungunya virus, kana iripo mumuenzaniso, inosunga kune Chikungunya conjugates.Iyo immunocomplex inozobatwa neiyo reagent pre-yakavharwa paM bhendi, ichigadzira burgundy ine ruvara rweM bhendi, ichiratidza Chikungunya virus IgM positive bvunzo mhedzisiro uye ichiratidza hutachiona hutsva.Kusavapo kwechero mabhendi ekuedzwa (G uye M) anoratidza mhedzisiro yakaipa.Muedzo une mukati mekutonga (C band) iyo inofanirwa kuratidza burgundy colored band ye immunocomplex yembudzi anti rabbit IgG/rabbit IgG-gold conjugate zvisinei nekusimudzirwa kwemavara pane chero maT bands.Zvikasadaro, mhedzisiro yebvunzo haishande uye mufananidzo unofanirwa kuongororwa nemumwe mudziyo.
